Author(s):
1. Sanja Stojanović, Medicinski fakultet Univerziteta u Nišu, Serbia
2. Mike Barbeck, BerlinAnalytix GmbH and University Medical Centre Rostock, Germany
3. Stevo Najman, Faculty of Medicine, University of Niš, Serbia

Abstract:
Collagen-based biomaterials are widely used in regenerative medicine and tissue engineering due to their favorable characteristics such as biocompatibility and good interaction with cells for the successful regeneration of soft and hard tissues. Collagen-based biomaterials can also be used in for wound healing. These biomaterials can be used alone or in combination with other biomaterials and biologically active agents, depending on the desired outcome and target tissue. The response of cells and tissues to collagen biomaterials depends on the characteristics of the biomaterials themselves, but also on the origin of the collagen and the method of its processing in order to produce the final biomaterial. The aim of this study was to examine the response of macrophages to the collagen membranes of the same species and tissue origin but produced by different manufacturing processes, in direct contact culture in vitro. Two collagen membranes originating from porcine pericardium, native and a new transparent membrane produced by chemical treatment of the native membrane during the manufacturing process, were tested on RAW 264.7 macrophages. The cells were incubated with collagen membranes as well as in control media for 72 hours. The response of macrophages was analyzed by assessing their function using the MTT, Neutral red and NBT assays, and the morphological characteristics of macrophages were also examined using the light microscopy method. The results obtained show that the response of macrophages to the presence of the two collagen membranes of the same origin but produced by different manufacturing processes differs, and that the manufacturing process affects the functional state of macrophages. The observed differences may indicate different behavior of these membranes after implantation as well as their application in different situations in the field of regenerative medicine. This research was supported by the Science Fund of the Republic of Serbia, #7617, Multilevel approach to study chronic wounds based on clinical and biological assessment with development of novel personalized therapeutic approaches using in vitro and in vivo experimental models – CHRONOWOUND and by the Ministry of Science, Technological Development and Innovation (Contracts No. 451-03-137/2025-03/200113).

Key words:
collagen membranes,manufacturing process,biomaterials,macrophages,regenerative medicine,wound healing

Thematic field:
SYMPOSIUM B - Biomaterials and nanomedicine

Date of abstract submission:
29.07.2025.

Conference:
Contemporary Materials 2025 - Savremeni Materijali

Applied paper from author