Author(s):
1. Sanja Stojanović, Medicinski fakultet Univerziteta u Nišu, Serbia
2. Danica Biberović, Neomedica d.o.o., Niš, Serbia, Serbia
3. Milena Radenković, University of Niš Faculty of Medicine, Serbia
4. Biljana Božić Nedeljković, Univerzitet u Beogradu - Biološki fakultet, Serbia
5. Jelena Živković, Serbia
6. Mike Barbeck, BerlinAnalytix GmbH and University Medical Centre Rostock, Germany
7. Stevo Najman, Faculty of Medicine, University of Niš, Serbia

Abstract:
Collagen membranes are widely used in the regeneration of both soft and hard tissues, and collagen-based biomaterials can also be used for wound healing. It is very important that these biomaterials are biocompatible but also that they interact with cells in a way that allows for adequate regeneration of the target tissue and the wound healing process. After implantation of biomaterials, the first cells to respond are macrophages, and their response depends on the inflammatory state of the tissue but also on the characteristics of the biomaterials themselves. The aim of this study was to examine the response of unstimulated and lipopolysaccharide (LPS)-stimulated macrophages to collagen membrane in direct contact culture in vitro. In this study, the collagen membrane derived from porcine pericardium was examined on RAW 264.7 macrophages. Cells were incubated with collagen membrane as well as in control media for 72 h. The response of macrophages was examined by evaluating their function using the MTT, Neutral red and NBT tests, and the morphological characteristics of macrophages were also examined using the light microscopy method. The obtained results indicate that the response of macrophages to the presence of a collagen membrane is different in the presence and absence of lipopolysaccharide, that is, it depends on whether the macrophages are stimulated by an inflammatory agent or not, but also that the presence of the collagen membrane itself can influence and change the functional state of stimulated macrophages. These results can be used as a strategy in tissue engineering to direct tissue and cell responses to biomaterials in order to achieve appropriate outcomes in tissue regeneration and the wound healing process. Acknowledgement: This research was supported by the Science Fund of the Republic of Serbia, #7617, Multilevel approach to study chronic wounds based on clinical and biological assessment with development of novel personalized therapeutic approaches using in vitro and in vivo experimental models – CHRONOWOUND and by the Ministry of Science, Technological Development and Innovation (Contracts No. 451-03-137/2025-03/200113 and 451-03-136/2025-03/200113).

Key words:
collagen membranes,biomaterials,macrophages,lipopolysaccharide,tissue regeneration,wound healing

Thematic field:
SYMPOSIUM B - Biomaterials and nanomedicine

Date of abstract submission:
29.07.2025.

Conference:
Contemporary Materials 2025 - Savremeni Materijali

Abstract