Author(s):
1. Jelena Najdanović, University of Niš Faculty of Medicine, Serbia
2. Vladimir Cvetković, University of Nis, Faculty of Sciences and Mathematics, ::Visegradska 33, Serbia
3. Sanja Stojanović, Faculty of Medicine, University of Niš, Niš, Serbia, Serbia
4. Milena Radenković, University of Niš Faculty of Medicine, Serbia
5. Jelena Živković, Serbia
6. Stevo Najman, Faculty of Medicine, University of Niš, Serbia

Abstract:
Novel approaches to bone tissue repair and regeneration frequently include the application of bone tissue engineering (BTE) methods. BTE methods involve the construction of implants composed of bone substitute biomaterials, source of growth factors and cells, in orthopic and ectopic models. The advantage of ectopic over orthopic models is that they exclude the influence of bone tissue components and also enable evaluation of the implants’ components on osteogenic process. The aim of this research was to examine the influence of implants composed of mice adipose-derived mesenchymal stem cells (ADSCs), platelet-rich plasma (PRP) and bone mineral matrix on ectopic osteogenic process. Two types of implants were prepared. The first type of implants contained in vitro cultivated, uninduced mice ADSCs, combined with PRP and loaded onto bone substitute biomaterial (bone mineral matrix – BMM) that was used as a carrier. The second type of implants contained only BMM (control group). The implantations were ectopic, subcutaneous. The implants were extracted after one early and one late post-implantation period in order to analyze the expression of osteogenic markers. The relative expression of gene that encodes osteopontin (gene Spp1) and immunoexpression of protein osteocalcin were examined. The Spp1 gene expression and osteocalcin immunoexpression were both significantly higher in the implants that contained cells and growth factors in comparison with the implants from control group. It was concluded that there is a promising potential for the application of implants constructed of in vitro cultivated, uninduced ADSCs, PRP and BMM in bone tissue regeneration. The authors would like to thank the Ministry of Education, Science and Technological Development of Republic of Serbia [Grant No: 451-03-65/2024-03/200113] for the financial support.

Key words:
adipose-derived mesenchymal stem cells,platelet-rich plasma,bone mineral matrix,ectopic osteogenic process,bone tissue regeneration

Thematic field:
SYMPOSIUM B - Biomaterials and nanomedicine

Date of abstract submission:
31.07.2024.

Conference:
Contemporary Materials 2024 - Savremeni Materijali

Applied paper from author Abstract