Author(s):
1. Milena Radenković, University of Niš Faculty of Medicine, Serbia
2. Sanja Stojanović, Faculty of Medicine, University of Niš, Niš, Serbia, Serbia
3. Stevo Najman, Faculty of Medicine, University of Niš, Serbia

Abstract:
Collagen membranes are widely used in the regeneration of soft and bone tissue when they can be applied alone, enriched with cells and certain physiological factors, or in combination with bone substitutes. As a natural polymer, collagen is a biocompatible material. Collagen can be isolated from various tissue compartments of different organisms, using different production procedures, which causes differences in the physico-chemical characteristics of collagen materials, but also tissue response after implantation. In addition, when placing biomaterials at the site of tissue injury, biomaterials come into contact with blood, which is another factor that can affect the dynamics of the interaction of tissues and implanted biomaterials. The aim of this study was to perform a comparative analysis of the tissue response to the collagen membrane with and without the addition of blood as a physiological factor, in order to predict the tissue response in realistic physiological conditions. The tissues response of to the collagen membrane of porcine origin with and without the addition of blood, as well as the changes in the membranes, were investigated in the mouse subcutaneous implantation model 3, 10 and 30 days after implantation. The explanted membranes and their interaction with the surrounding tissue were analyzed by histological method as well as the application of scanning electron microscopy. A difference was observed in the reaction of the surrounding tissue, which is reflected in the different number of cells and the presence of different cell populations, as well as the pattern of cellular infiltration, at all observed time points The obtained results indicate that the tissue response and the behavior of the implanted collagen membrane differ depending on whether blood is added to the membrane or not, which may have a great practical significance in the clinical application of collagen membranes.

Key words:
collagen membranes,implantation,tissue response,blood,collagen membranes

Thematic field:
SYMPOSIUM B - Biomaterials and nanomedicine

Date of abstract submission:
12.08.2023.

Conference:
Contemporary Materials 2023 - Savremeni materijali

Applied paper from author