Author(s):
1. Sanja Stojanović, University of Niš Faculty of Medicine, Serbia
2. Jelena Najdanović, University of Niš Faculty of Medicine, Serbia
3. Žarko Mitić, University of Niš Faculty of Medicine, Serbia
4. Perica Vasiljević, University of Nis, Faculty of Sciences and Mathematics, ::Visegradska 33, Serbia
5. Nenad Ignjatović, Institute of Technical Sciences of the Serbian Academy of Sciences and Arts, Belgrade, Serbia, Serbia
6. Stevo Najman, Faculty of Medicine, University of Niš, Serbia

Abstract:
Testing biomaterials intended for use in regenerative medicine on cell models in vitro is a necessary first step in order to assess the biocompatibility of biomaterials as well as their biofunctionality. There are different methods of testing biomaterials, as well as different cell models on which testing is performed. The selection of an adequate cell model is made based on the type of biomaterial and its potential application, and testing is performed first using standard tests such as cytotoxicity and genotoxicity tests, and then other biological characteristics are examined such as, for example, the ability of biomaterials to induce the differentiation of cells into a certain type or the ability to activate macrophages towards a certain phenotype and the induction of their response in vitro, and many others. This paper will present the results of our research in the field of characterization of biomaterials and examination on cell models in vitro. We examined biomaterials intended for the regeneration of hard tissues, e.g. materials based on hydroxyapatite, calcium phosphate, of natural and synthetic origin, as well as biomaterials for the regeneration of soft tissues, e.g. materials based on collagen or other polymers of both natural and synthetic origin. In order to improve the biofunctionality of biomaterials, bioactive substances or their combinations are often added, and the biomaterials can be in different forms, in the form of scaffolds, in the form of granules, nano- or micro-dimensions, membranes and many others, whereby the shape of the material also affects the selection of the cell model used for testing. To characterize bimaterials, we used methods such as SEM, EDX, FTIR and others. In addition to cytotoxicity and genotoxicity tests, we performed proliferation, migration, differentiation, phagocytosis and many other tests, and we tested biomaterials both in direct contact with cells and in indirect contact. We also examined changes in biomaterials after interaction with cells, as well as the behavior of biomaterials in various conditions in vitro.

Key words:
biomaterials,cells,in vitro,material characterization

Thematic field:
SYMPOSIUM B - Biomaterials and nanomedicine

Date of abstract submission:
16.08.2022.

Conference:
Contemporary Materials 2022 - Savremeni materijali

Abstract